Document 0558
 DOCN  M9480558
 TI    Antisense oligonucleotide complementary to endogenous retroviral MCF env
       gene inhibits both BFU-E and CFU-S colony formation in mice.
 DT    9410
 AU    Chernukhin IV; Khaldoyanidi SK; Dikovskaya DV; Svinarchuk FP; Vlasov VV;
       Gaidul KV; Institute of Clinical Immunology, Novosibirsk, Russian;
       Federation.
 SO    FEBS Lett. 1994 Jul 11;348(2):197-200. Unique Identifier : AIDSLINE
       MED/94307424
 AB    A possible biologic activity of endogenously expressed env sequence of
       retroviral mink cell focus-forming virus (MCF) genome for hematopoietic
       colony formation was studied in mice. Antisense 20-mer complementary to
       MCF env sequence was used to detect the result of blockage of this gene
       translation on the potency of marrow cells to form colonies of erythroid
       (BFU-E), myeloid granulocyte-macrophage (CFU-GM), and stem cell (day 11
       CFU-S) hematopoietic compartments. A large relative decrease in BFU-E
       number was found in bone marrow cell cultures preincubated with
       antisense oligonucleotide during 4 h, whereas CFU-GM colonies remained
       unaffected. A marked reduction of CFU-S colony formation was also
       registered under antisense oligomer influence. Following a decreased
       proliferation of erythroid progenitors, we suggest the mechanism by
       which antisense oligonucleotide could cause the loss of colony
       formation. Taken together, these data allow to propose that the
       expression of this gene is naturally significant for hematopoietic
       progenitor activity exerting some property of env gene products to
       regulate the growth of erythroid and multilineage hematopoietic
       precursors.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cell Division/DRUG EFFECTS
       Cells, Cultured  Colony-Forming Units Assay  *Genes, env  Hematopoietic
       Stem Cells/CYTOLOGY/*DRUG EFFECTS  Male  Mice  Mice, Inbred C57BL  Mink
       Cell Focus-Inducing Viruses/*GENETICS  Molecular Sequence Data
       Oligonucleotides, Antisense/GENETICS/*PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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